Recent Publications
2009
Blum G, Weimer RM, Edgington LE, Adams W, Bogyo M. Comparative assessment of substrates and activity based probes as tools for non-invasive optical imaging of cysteine protease activity. PLoS One(2009) July 28; 4(7): e6374 pdf
Puri AW, Bogyo M. Using Small Molecules to Dissect Mechanisms of Microbial Pathogenesis. ACS Chem. Biol. (2009) July 16. [Epub ahead of print]. pdf about the author
Lee JT, Chen DY, Yang Z, Ramos AD, Hsieh JJD, Bogyo M. Design, synthesis, and evaluation of Taspase1 inhibitors. Bioorg. Med. Chem. Let. (2009) 19:5086-5090 pdf
Edgington LE, Berger AB, Blum G, Albrow VE, Paulick MG, Lineberry N, Bogyo M. Noinvasive optical imaging of apoptosis by caspase-targeted activity based probes. Nat Med (2009) 15:967-73 pdf Stanford Press Release
Larson ET, Parussini F, Huynh MH, Giebel JD, Kelley AM, Zhang L, Bogyo M, Merritt EA, Carruthers VB. Toxoplasma gondii cathepsin l is the primary target of the invasion inhibitory compound LHVS. J. Biol. Chem. (2009) July 17. [Epub ahead of print]. pdf
Shen A, Lupardus PJ, Albrow VE, Guzzetta A, Powers JC, Garcia KC, Bogyo M. Mechanistic and structural insights into the proteolytic activation of Vibrio cholerae MARTX toxin. Nat Chem Biol. (2009) 5:469-478. pdf
Chang SH, Kanasaki K, Gocheva V, Blum G, Harper J, Moses MA, Shih SC, Nagy JA, Joyce J, Bogyo M, Kalluri R, Dvorak HF. VEGF-A induces angiogenesis by perturbing the cathepsin-cysteine protease inhibitor balance in venules, causing basement membrane degradation and mother vessel formation. Cancer Res. (2009) 69:4537-44. pdf
Beckham SA, Piedrafita D, Phillips CI, Samarawickrema N, Law RH, Smooker PM, Quinsey NS, Irving JA, Greenwood D, Verhelst SH, Bogyo M, Turk B, Coetzer TH, Wijeyewickrema LC, Spithill TW, Pike RN. A major cathepsin B protease from the liver fluke Fasciola hepatica has atypical active site features and a potential role in the digestive tract of newly excysted juvenile parasites. Int J Biochem Cell Biol. (2009) 41:1601-12. pdf
Barbero S, Mielgo A, Torres V, Teitz T, Shields DJ, Mikolon D, Bogyo M, Barilà D, Lahti JM, Schlaepfer D, Stupack DG. Caspase-8 association with the focal adhesion complex promotes tumor cell migration and metastasis. Cancer Res. (2009) 69:3755-63. pdf
Cavallo-Medved D, Rudy D, Blum G, Bogyo M, Caglic D, Sloane BF. Live-cell imaging demonstrates extracellular matrix degradation in association with active cathepsin B in caveolae of endothelial cells during tube formation. Exp Cell Res. (2009) 315:1234-46. pdf
Obermajer N, Jevnikar Z, Doljak B, Sadaghiani AM, Bogyo M, Kos J. Cathepsin X-mediated beta2 integrin activation results in nanotube outgrowth. Cell Mol Life Sci. (2009) 66:1126-34. pdf
Pungercar JR, Caglic D, Sajid M, Dolinar M, Vasiljeva O, Pozgan U, Turk D, Bogyo M, Turk V, Turk B. Autocatalytic processing of procathepsin B is triggered by proenzyme activity. FEBS J. (2009) 276:660-8. pdf
Liz MA, Fleming CE, Nunes AF, Almeida MR, Mar FM, Choe Y, Craik CS, Powers JC, Bogyo M, Sousa MM. Substrate specificity of transthyretin: identification of natural substrates in the nervous system. Biochem J. (2009) Biochem J. 2009 Apr 15;419(2):467-74. pdf
Bogyo M. Metabolomics cuts to the chase to chase the cuts. Nat Chem Biol. (2009) 5:5-6. pdf
2008
Fonovic M, Bogyo M. Activity-based probes as a tool for functional proteomic analysis of proteases. Expert Rev Proteomics. (2008) 5:721-30. pdf
Lupardus PJ, Shen A, Bogyo M, Garcia KC.Small molecule-induced allosteric activation of the Vibriocholerae RTX cysteine protease domain. Science. (2008) 322:265-8. pdf Sup. Mat.
Shen A, Bogyo M. Friend or Foe? Turning a Host Defense Protein Into a Pathogen's Accomplice. Chem Biol.(2008) 15:879-80. pdf
Kaschani F, Verhelst SH, van Swieten PF, Verdoes M, Wong CS, Wang Z, Kaiser M, Overkleeft HS, Bogyo M, van der Hoorn RA. Minitags for small molecules: detecting targets of reactive small molecules in living plant tissues using 'click-chemistry' Plant J. (2008) 57:373-85. pdf
Schurigt U, Sevenich L, Vannier C, Gajda M, Schwinde A, Werner F, Stahl A, von Elverfeldt D, Becker AK, Bogyo M, Peters C, Reinheckel T. Trial of the cysteine cathepsin inhibitor JPM-OEt on early and advanced mammary cancer stages in the MMTV-PyMT-transgenic mouse model. Biol Chem. (2008) 389:1067-74. pdf
Obermajer N, Svajger U, Bogyo M, Jeras M, Kos J. Maturation of dendritic cells depends on proteolytic cleavage by cathepsin X. J Leukoc Biol. (2008) 84:1306-14. pdf
Jevnikar Z, Obermajer N, Bogyo M, Kos J. The role of cathepsin X in the migration and invasiveness of T lymphocytes. J Cell Sci. (2008) 121:2652-61. pdf
Yang Z, Fonovic M, Verhelst SH, Blum G and Bogyo M. Evaluation of alpha,beta-unsaturated ketone-based probes for papain-family cysteine proteases. Bioorg Med Chem. (2008) Mar 2. [Epub ahead of print]. pdf.
Paulick MG, Bogyo M. Application of activity-based probes to the study of enzymes involved in cancer progression. Curr Opin Genet Dev. (2008) 18:97-106. pdf.
Arastu-Kapur S, Ponder EL, Fonovi? UP, Yeoh S, Yuan F, Fonovic M, Grainger M, Phillips CI, Powers JC, and Bogyo M. Identification of proteases that regulate erythrocyte rupture by the malaria parasite Plasmodium falciparum. Nature Chemical Biology (2008) 4:203-213. pdf. News and Views pdf
2007
Ponder EL and Bogyo M. Ubiquitin-like modifiers and their deconjugating enzymes in medically important parasitic protozoa.Eukaryot Cell. (2007) 6:1943-1952. pdf.
Goulet B, Sansregret L, Leduy L, Bogyo M, Weber E, Chauhan SS, Nepveu A. Increased expression and activity of nuclear cathepsin L in cancer cells suggests a novel mechanism of cell transformation. Mol Cancer Res. (2007) 5:899-907. pdf.
Blum, G., von Degenfeld, G., Merchant, M.J., Blau, H.M., and Bogyo, M. Noinvasive optical imaging of cysteine protease activity using fluorescently quenched activity-based probes. (2007) Nature Chemical Biology. 3: 668-677. pdf.
Fonovic M, Verhelst SH, Sorum MT, Bogyo M. Proteomic evaluation of chemically cleavable activity based probes. (2007) Mol Cell Proteomics. 6: 1761-1770. pdf
Bell-McGuinn KM, Garfall AL, Bogyo M, Hanahan D, Joyce JA. Inhibition of cysteine cathepsin protease activity enhances chemotherapy regimens by decreasing tumor growth and invasiveness in a mouse model of multistage cancer. (2007) Cancer Res. 67:7378-85. pdf
Burster T, Giffon T, Dahl ME, Bjorck P, Bogyo M, Weber E, Mahmood K, Lewis DB, Mellins ED. Influenza A virus elevates active cathepsin B in primary murine DC. (2007) Int Immunol. 19:645-55. pdf
Rose PP, Bogyo M, Moses AV, Fruh K. Insulin-like growth factor II receptor-mediated intracellular retention of cathepsin B is essential for transformation of endothelial cells by Kaposi's sarcoma-associated herpesvirus. (2007) J Virol. 81:8050-62. pdf
Sadaghiani, A.M.,.Verhelst, S.H.L., Gocheva, V., Hill, K., Majerova, E., Stinson, S. Joyce, J.A. and Bogyo, M. Design, synthesis and evaluation of in vivo potency and selectivity of epoxysuccinyl-based inhibitors of papain family cysteine proteases. (2007) Chem Biol 14:499-511. pdf
Sojka D, Hajdusek O, Dvorak J, Sajid M, Franta Z, Schneider EL, Craik CS, Vancova M, Buresova V, Bogyo M, Sexton KB, McKerrow JH, Caffrey CR, Kopacek P. IrAE - An asparaginyl endopeptidase (legumain) in the gut of the hard tick Ixodes ricinus. (2007) Int J Parasitol. 37:713-724.
Cuerrier D, Moldoveanu T, Campbell RL, Kelly J, Yoruk B, Verhelst SH, Greenbaum D, Bogyo M, Davies PL. Development of calpain-specific inactivators by screening of positional-scanning epoxide libraries. (2007) J Biol Chem. 282:9600-96011. pdf
Bogyo, M and Cravatt BF. Genomics and proteomics From genes to function: advances in applications of chemical and systems biology. (2007) Curr Opin Chem Biol. 11:1-3. pdf
Teo CF, Zhou XW, Bogyo, M, Carruthers VB. Cysteine protease inhibitors block Toxoplasma gondii microneme secretion and cell invasion. (2007) Antimicrob Agents Chemother. 51, 679-88. pdf
Verhelst, S.H.L., Fonovic, M. and Bogyo, M. A Mild Chemically Cleavable Linker System for Functional Proteomic Applications. (2007) Angew. Chemie. 46:1284-1286. pdf
Fonovic, M. and Bogyo, M. Activity Based Probes for Proteases: Applications to Biomarker Discovery, Molecular Imaging and Drug Screening. (2007) Current Pharmaceutical Design. 13:253-261 pdf
Sadaghiani, A.M., Verhelst, S.H.L., and Bogyo, M.Tagging and Detection Strategies for Activity-Based Proteomics. (2007) Cur Opin Chem. Bio. 11:20-28. pdf
Sexton, K.B., Witte, M.D., Blum, G., and Bogyo, M. Design of cell permeable, fluorescent activity based probes of the lysosomal cysteine protease asparaginyl endopeptidase (AEP)/legumain. (2007) Bioorg. Med. Chem. Let. 17:649-653. pdf
Sexton, K.B., Kato, D., Berger, A.B., Fonovic, M., Verhelst, S.H.L. and Bogyo, M. Specificity if aza-peptide electrophile activity based probes of caspases. (2007) Cell Death Differ 14:727-732. pdf
2006
Berger, A.B., Sexton, K.B., and Bogyo, M. Commonly used caspase inhibitors designed based on substrate specificity profiles lack selectivity. (2006) Cell Research. 16:961-963. pdf
Sadaghiani, A. M.,Verhelst, S. H. L. and Bogyo, M. Solid-Phase Methods for the Preparation of Epoxysuccinate-Based Inhibitors of Cysteine Proteases. (2006) J. Comb. Chem. 8: 802-804. pdf Sup Mat
Pandey, K.C., Singh, N., Arastu-Kapur, S., Bogyo, M. and Rosenthal P.J. Falstatin, a Cysteine Protease Inhibitor of Plasmodium falciparum, Facilitates Erythrocyte Invasion. (2006) PloS Pathogens. 2: e117 pdf
Denault, J.-B., Bekes, M., Scott, F.L., Sexton, K.B., Bogyo, M. and Salvesen, G.S., Engineered hybrid dimers: tracking the activation pathway of caspase-7. (2006) Molecular Cell. 23:523-533. pdf
Berger, A.B., Witte, M.D., Denault, J.-B., Sadaghiani, A.M., Sexton, K.B., Salvesen, G.S. and Bogyo, M. Identification of early intermediates of caspase activation using selective inhibitors and activity-based probes . (2006) Molecular Cell. 23:509-521. pdf
Vasiljeva, O., Papazoglou, A., Kruger, A., Brodoefel, H., Korovin, M., Deussing, J., Augustin, N., Nielsen, B.S., Almholt, K., Bogyo, M., Peters, C. and Reinheckel, T. Tumor cell-derived and macrophage-derived cathepsin B promotes progression and lung metastasis of mammary cancer. (2006) Cancer Res. 66:5242-5250. pdf
Yuan, F., Verhelst, S., Blum, G., Coussens, L. and Bogyo, M. A selective activity-based probe for the papain family cysteine protease dipeptidyl peptidase I/cathepsin C. (2006) J. Am. Chem. Soc. 128:5616-5617. pdf
Verhelst, S.H.L., Witte, M.D., Arastu-KApur, S., Fonovic M., and Bogyo, M. Novel aza peptide inhibitors and active site probes of papain family cysteine proteases. (2006) ChemBioChem, epub ahead of print. pdf.
Pan Z, Jeffery DA, Chehade K, Beltman J, Clark JM, Grothaus P, Bogyo M, and Baruch A. Development of activity-based probes for trypsin-family serine proteases. (2006) Bioorg Med Chem Lett. 16:2882-2885. pdf
Choe Y., Leonetti F., Greenbaum D.C., Lecaille F., Bogyo M., Bromme D., Ellman J.A. andCraik C.S. Substrate profiling of cysteine proteases using a combinatorial peptide library identifies functionally unique specificities.(2006) J Biol Chem. 281:12824-12832. pd
2005
Kato, D., Verhelst, S.H.L., Sexton, K.B. and Bogyo, M. A General Solid Phase Method for the Preparation of Diverse Azapeptide Probes Directed Against Cysteine Proteases. (2005) Organic Letters, 7:5649-5652.pdf
Blum, G., Mullins, S.R., Keren, K., Fonovic, M., Jedeszko, C., Rice, M.J., Sloane, B.F. and Bogyo, M. Dynamic imaging of protease activity with fluorescently quenched activity-based probes. (2005) Nature Chemical Biology, 1:203-209.pdf Nature Chemical Biology website News and Views
Kato, D., Boatright, K.M., Berger, A.B., Nazif, T., Blum, G., Ryan, C., Chehade, K.A.H., Salvesen, G., and Bogyo, M. Activity based probes that target diverse cysteine protease families. (2005) Nature Chemical Biology 1:33-38. pdf Nature Chemical Biology website
Verhelst, S.H.L, and Bogyo, M. Dissecting protein function using chemical proteomic methods. (2005) QSAR in press.
Phillips, C.I., and Bogyo, M. Proteomics meets microbiology: technical advances in the global mapping of protein expression and function. (2005) Cellular Microbiology 7(8):1061-76. Abstract pdf
Verhelst S.H.L. and Bogyo, M. Solid-Phase Synthesis of Double-Headed Epoxysuccinyl Activity-Based Probes for Selective Targeting of Papain Family Cysteine Proteases. (2005) ChemBioChem 6(5):824-827. Abstract pdf
Verhelst S.H.L. and Bogyo, M. Chemical proteomics applied to target identification and drug discovery. (2005) Biotechniques 38(2):175-7. Abstract pdf
Chehade, K.A.H., Baruch, A., Verhelst, S.H.L. and Bogyo, M.An improved preparation of the activity-based probe JPM-OEt and in situ applications. (2005) Synthesis 2: 240-244.
Borodovsky, A., Ovaa. H., Meester, W.J., Venanzi, E.S., Bogyo, M., Hekking B.G., Ploegh, H.L., Kessler, B.M. and Overkleeft, H.S. Small-molecule inhibitors and probes for ubiquitin- and ubiquitin-like-specific proteases. (2005) Chembiochem 6(2):287-91. Abstract pdf
2004
Berger, A.B., Vitorino, P.M., Bogyo, M. Activity-based protein profiling: applications to biomarker discovery, in vivo imaging and drug discovery. (2004) Am J Pharmacogenomics 4(6):371-81. Abstract pdf
Snipas, SJ, Wildfang E, Nazif T, Christensen L, Boatright KM, Bogyo M, Stennicke HR, Salvesen GS Characteristics of the caspase-like catalytic domain of human paracaspase. (2004) Biol Chem 385(11):1093-8. Abstract pdf
van der Hoorn RA, Leeuwenburgh MA, Bogyo M, Joosten MH, Peck SC Activity profiling of papain-like cysteine proteases in plants. (2004) Plant Physiol 135(3):1170-8. Abstract pdf
Oleksy A, Golonka E, Banbula A, Szmyd G, Moon J, Kubica M, Greenbaum D, Bogyo M, Foster TJ, Travis J, Potempa J Growth phase-dependent production of a cell wall-associated elastinolytic cysteine proteinase by Staphylococcus epidermidis. (2004) Biol Chem 385(6):525-35. Abstract pdf
Hook V, Yasothornsrikul S, Greenbaum D, Medzihradszky KF, Troutner K, Toneff T, Bundey R, Logrinova A, Reinheckel T, Peters C, Bogyo M Cathepsin L and Arg/Lys aminopeptidase: a distinct prohormone processing pathway for the biosynthesis of peptide neurotransmitters and hormones. (2004) Biol Chem 385(6):473-80. Abstract pdf
Eksi S, Czesny B, Greenbaum DC, Bogyo M, Williamson KC Targeted disruption of Plasmodium falciparum cysteine protease, falcipain 1, reduces oocyst production, not erythrocytic stage growth. (2004) Mol Microbiol 53(1):243-50. Abstract pdf
Yasuda Y, Li Z, Greenbaum D, Bogyo M, Weber E, Bromme D Cathepsin V, a novel and potent elastolytic activity expressed in activated macrophages. (2004) J Biol Chem 279(35):36761-70. Abstract pdf
Joyce JA, Baruch A, Chehade K, Meyer-Morse N, Giraudo E, Tsai FY, Greenbaum DC, Hager JH, Bogyo M, Hanahan D Cathepsin cysteine proteases are effectors of invasive growth and angiogenesis during multistage tumorigenesis. (2004) Cancer Cell 5(5):443-53. Abstract pdf
Goulet B, Baruch A, Moon NS, Poirier M, Sansregret LL, Erickson A, Bogyo M, Nepveu A A cathepsin L isoform that is devoid of a signal peptide localizes to the nucleus in S phase and processes the CDP/Cux transcription factor. (2004) Mol Cell 14(2):207-19. Abstract pdf
Medzihradszky KF, Darula Z, Perlson E, Fainzilber M, Chalkley RJ, Ball H, Greenbaum D, Bogyo M, Tyson DR, Bradshaw RA, Burlingame AL O-sulfonation of serine and threonine: mass spectrometric detection and characterization of a new posttranslational modification in diverse proteins throughout the eukaryotes. (2004) Mol Cell Proteomics 3(5):429-40. Abstract pdf
Baruch A, Jeffery DA, Bogyo M Enzyme activity--it's all about image. (2004) Trends Cell Biol 14(1):29-35. Abstract pdf
Li Z, Yasuda Y, Li W, Bogyo M, Katz N, Gordon RE, Fields GB, Bromme D Regulation of collagenase activities of human cathepsins by glycosaminoglycans. (2004) J Biol Chem 279(7):5470-9. Abstract pdf
2003
Sajid M, McKerrow JH, Hansell E, Mathieu MA, Lucas KD, Hsieh I, Greenbaum D, Bogyo M, Salter JP, Lim KC, Franklin C, Kim JH, Caffrey CR Functional expression and characterization of Schistosoma mansoni cathepsin B and its trans-activation by an endogenous asparaginyl endopeptidase. (2003) Mol Biochem Parasitol 131(1):65-75. Abstract pdf
Yasothornsrikul S, Greenbaum D, Medzihradszky KF, Toneff T, Bundey R, Miller R, Schilling B, Petermann I, Dehnert J, Logvinova A, Goldsmith P, Neveu JM, Lane WS, Gibson B, Reinheckel T, Peters C, Bogyo M, Hook V Cathepsin L in secretory vesicles functions as a prohormone-processing enzyme for production of the enkephalin peptide neurotransmitter. (2003) Proc Natl Acad Sci 100(16):9590-5. Abstract pdf
Rozman-Pungercar J, Kopitar-Jerala N, Bogyo M, Turk D, Vasiljeva O, Stefe I, Vandenabeele P, Bromme D, Puizdar V, Fonovic M, Trstenjak-Prebanda M, Dolenc I, Turk V, Turk B Inhibition of papain-like cysteine proteases and legumain by caspase-specific inhibitors: when reaction mechanism is more important than specificity. (2003) Cell Death Differ 10(8):881-8. Abstract pdf
Wang CC, Bozdech Z, Liu CL, Shipway A, Backes BJ, Harris JL, Bogyo M Biochemical analysis of the 20S proteasome of Trypanosoma brucei. (2003) J Biol Chem 278(18):15800-8. Abstract pdf
Mikolajczyk J, Boatright KM, Stennicke HR, Nazif T, Potempa J, Bogyo M, Salvesen GS Sequential autolytic processing activates the zymogen of Arg-gingipain. (2003) J Biol Chem 278(12):10458-64. Abstract pdf
Kessler B, Hong X, Petrovic J, Borodovsky A, Dantuma NP, Bogyo M, Overkleeft HS, Ploegh H, Glas R Pathways accessory to proteasomal proteolysis are less efficient in major histocompatibility complex class I antigen production. (2003) J Biol Chem 278(12):10013-21. Abstract pdf
Jeffery DA, Bogyo M Chemical proteomics and its application to drug discovery. (2003) Curr OpinBiotechnol 14(1):87-95. Abstract pdf
2002
Greenbaum DC, Baruch A, Grainger M, Bozdech Z, Medzihradszky KF, Engel J, DeRisi J, Holder AA, Bogyo M A role for the protease falcipain 1 in host cell invasion by the human malaria parasite. (2002) Science 298(5600):2002-6. Abstract pdf
Greenbaum DC, Arnold WD, Lu F, Hayrapetian L, Baruch A, Krumrine J, Toba S, Chehade K, Bromme D, Kuntz ID, Bogyo M Small molecule affinity fingerprinting. A tool for enzyme family subclassification, target identification, and inhibitor design. (2002) Chem Biol 9(10):1085-94. Abstract pdf
Groll M, Nazif T, Huber R, Bogyo M Probing structural determinants distal to the site of hydrolysis that control substrate specificity of the 20S proteasome. (2002) Chem Biol 9(5):655-62. Abstract pdf
Mathieu MA, Bogyo M, Caffrey CR, Choe Y, Lee J, Chapman H, Sajid M, Craik CS, McKerrow JH Substrate specificity of schistosome versus human legumain determined by P1-P3 peptide libraries. (2002) Mol Biochem Parasitol 121(1):99-105. Abstract pdf
Bogyo M, Wang EW Proteasome inhibitors: complex tools for a complex enzyme. (2002) Curr Top Microbiol Immunol 268:185-208. Abstract pdf
Greenbaum D, Baruch A, Hayrapetian L, Darula Z, Burlingame A, Medzihradszky KF, Bogyo M Chemical approaches for functionally probing the proteome. (2002) Mol Cell Proteomics 1(1):60-8. Abstract pdf
2001
Caffrey CR, Hansell E, Lucas KD, Brinen LS, Alvarez Hernandez A, Cheng J, Gwaltney SL 2nd, Roush WR, Stierhof YD, Bogyo M, Steverding D, McKerrow JH Active site mapping, biochemical properties and subcellular localization of rhodesain, the major cysteine protease of Trypanosoma brucei rhodesiense. (2001) Mol Biochem Parasitol 118(1):61-73. Abstract pdf
Baruch A, Greenbaum D, Levy ET, Nielsen PA, Gilula NB, Kumar NM, Bogyo M Defining a link between gap junction communication, proteolysis, and cataract formation. (2001) J Biol Chem 276(31):28999-9006. Abstract pdf
Li J, Gao X, Ortega J, Nazif T, Joss L, Bogyo M, Steven AC, Rechsteiner M Lysine 188 substitutions convert the pattern of proteasome activation by REGgamma to that of REGs alpha and beta. (2001) EMBO J 20(13):3359-69. Abstract pdf
Nazif T, Bogyo M Global analysis of proteasomal substrate specificity using positional-scanning libraries of covalent inhibitors. (2001) Proc Natl Acad Sci 98(6):2967-72. Abstract pdf
2000
Weihofen A, Lemberg MK, Ploegh HL, Bogyo M, Martoglio B Release of signal peptide fragments into the cytosol requires cleavage in the transmembrane region by a protease activity that is specifically blocked by a novel cysteine protease inhibitor. (2000) J. Biol. Chem. 275(40):30951-6. Abstract pdf
Wang EW, Kessler BM, Borodovsky A, Cravatt BF, Bogyo M, Ploegh HL, Glas R Integration of the ubiquitin-proteasome pathway with a cytosolic oligopeptidase activity. (2000) Proc Natl Acad Sci 97(18):9990-5. Abstract pdf
Greenbaum D, Medzihradszky KF, Burlingame A, Bogyo M Epoxide electrophiles as activity-dependent cysteine protease profiling and discovery tools. (2000) Chem Biol 7(8):569-81. Abstract pdf
Caffrey CR, Mathieu MA, Gaffney AM, Salter JP, Sajid M, Lucas KD, Franklin C, Bogyo M, McKerrow JH Identification of a cDNA encoding an active asparaginyl endopeptidase of Schistosoma mansoni and its expression in Pichia pastoris. (2000) FEBS Lett 466(2-3):244-8. Abstract pdf
Bogyo M, Verhelst S, Bellingard-Dubouchaud V, Toba S, Greenbaum D Selective targeting of lysosomal cysteine proteases with radiolabeled electrophilic substrate analogs. (2000) Chem Biol 7(1):27-38. Abstract pdf supplemental materials
1999
Schmidtke G, Holzhutter HG, Bogyo M, Kairies N, Groll M, de Giuli R, Emch S, Groettrup M How an inhibitor of the HIV-I protease modulates proteasome activity. (1999) J Biol Chem 274(50):35734-40. Abstract pdf
Selzer PM, Pingel S, Hsieh I, Ugele B, Chan VJ, Engel JC, Bogyo M, Russell DG, Sakanari JA, McKerrow JH Cysteine protease inhibitors as chemotherapy: lessons from a parasite target. (1999) Proc Natl Acad Sci 96(20):11015-22. Abstract pdf
1998
Bogyo M, Ploegh HL Antigen presentation. A protease draws first blood. (1998) Nature 396(6712):625-627. Abstract pdf
Bogyo M, Shin S, McMaster JS, Ploegh HL Substrate binding and sequence preference of the proteasome revealed by active-site-directed affinity probes. (1998) Chem Biol 5(6):307-20. Abstract pdf
Glas R, Bogyo M, McMaster JS, Gaczynska M, Ploegh HL A proteolytic system that compensates for loss of proteasome function. (1998) Nature 392(6676):618-22. Abstract pdf
Ruepp A, Eckerskorn C, Bogyo M, Baumeister W Proteasome function is dispensable under normal but not under heat shock conditions in Thermoplasma acidophilum. (1998) FEBS Lett 425(1):87-90. Abstract pdf
1997
Bogyo M, McMaster JS, Gaczynska M, Tortorella D, Goldberg AL, Ploegh H Covalent modification of the active site threonine of proteasomal beta subunits and the Escherichia coli homolog HslV by a new class of inhibitors. (1997) Proc Natl Acad Sci 94(13):6629-34. 9316392 Abstract pdf
Bogyo M, Gaczynska M, Ploegh HL Proteasome inhibitors and antigen presentation. (1997) Biopolymers 94(13):6629-34. Abstract pdf
1996
Wiertz EJ, Tortorella D, Bogyo M, Yu J, Mothes W, Jones TR, Rapoport TA, Ploegh HL Sec61-mediated transfer of a membrane protein from the endoplasmic reticulum to the proteasome for destruction. (1996) Nature 384(6608):432-8. Abstract pdf
Wiertz EJ, Jones TR, Sun L, Bogyo M, Geuze HJ, Ploegh HL The human cytomegalovirus US11 gene product dislocates MHC class I heavy chains from the endoplasmic reticulum to the cytosol. (1996) Cell 84(5):769-79. Abstract pdf