Recent Publications


2016

Lentz CS, Ordonez AA, Kasperkiewicz P, La Greca F, O'Donoghue AJ, Schulze CJ, Powers JC, Craik CS, Drag M, Jain SK, Bogyo M. Design of selective substrate and activity-based probes for Hydrolase Important for Pathogenesis 1 (HIP1) from Mycobacterium tuberculosis. ACD Infect. Dis. 2016 DOI: 10.1021/acsinfecdis.6b00092. pdf

Sanman LE, van der Linden WA, Verdoes M, Bogyo M. Bifunctional Probes of Cathepsin Protease Activity and pH Reveal Alterations in Endolysosomal pH during Bacterial Infection. Cell Chem Biol. 2016 23(7):793-804. pdf

van der Linden, W.A., Schulze, C.J., Herbert, A.S., Krause, T.B., Wirchnianski, A.A., Dye, J.M., Chandran, K., Bogyo M. Cysteine Cathepsins as anti-ebola agents. ACS Infect Dis. 2016 2(3):173-179. pdf

Li H, Bogyo M, da Fonseca PC. The cryo-EM structure of the Plasmodium falciparum 20S proteasome and its use in the fight against malaria. FEBS J. 2016 Jun 11. doi: 10.1111/febs.13780. [Epub ahead of print] pdf

Withana NP, Saito T, Ma X, Garland M, Liu C, Kosuge H, Amsallem M, Verdoes M, Ofori LO, Fischbein M, Arakawa M, Cheng Z, McConnell MV, Bogyo M. Dual Modality Activity Based Probes as Molecular Imaging Agents for Vascular Inflammation. J Nucl Med. 2016 May 19. pii: jnumed.115.171553. [Epub ahead of print] pdf

Sensarn S, Zavaleta CL, Segal E, Rogalla S, Lee W, Gambhir SS, Bogyo M, Contag CH. A Clinical Wide-Field Fluorescence Endoscopic Device for Molecular Imaging Demonstrating Cathepsin Protease Activity in Colon Cancer. Mol Imaging Biol. 2016 May 6. [Epub ahead of print] pdf

Edgington-Mitchell LE, Bogyo M. Detection of Active Caspases During Apoptosis Using Fluorescent Activity-Based Probes. Methods Mol Biol. 2016 1419:27-39. pdf

Sanman LE, Qian Y, Eisele NA, Ng TM, van der Linden WA, Monack DM, Weerapana E, Bogyo M. Disruption of glycolytic flux is a signal for inflammasome signaling and pyroptotic cell death. Elife. 2016 5:e13663. pdf

Garland M, Yim JJ, Bogyo M. A Bright Future for Precision Medicine: Advances in Fluorescent Chemical Probe Design and Their Clinical Application. Cell Chem Biol. 2016 23(1):122-36. pdf

Li H, O'Donoghue AJ, van der Linden WA, Xie SC, Yoo E, Foe IT, Tilley L, Craik CS, da Fonseca PC, Bogyo M. Structure- and function-based design of Plasmodium-selective proteasome inhibitors. Nature. 2016 530(7589):233-6. pdf

Withana NP, Ma X, McGuire HM, Verdoes M, van der Linden WA, Ofori LO, Zhang R, Li H, Sanman LE, Wei K, Yao S, Wu P, Li F, Huang H, Xu Z, Wolters PJ, Rosen GD, Collard HR, Zhu Z, Cheng Z, Bogyo M.Non-invasive Imaging of Idiopathic Pulmonary Fibrosis Using Cathepsin Protease Probes. Sci Rep. 2016 6:19755. pdf

Withana NP, Garland M, Verdoes M, Ofori LO, Segal E, Bogyo M. Labeling of active proteases in fresh-frozen tissues by topical application of quenched activity-based probes. Nat Protoc. 2016 11(1):184-91. pdf

Lauterwasser EM, Fontaine SD, Li H, Gut J, Katneni K, Charman SA, Rosenthal PJ, Bogyo M, Renslo AR. Trioxolane-Mediated Delivery of Mefloquine Limits Brain Exposure in a Mouse Model of Malaria. ACS Med Chem Lett. 2015 6(11):1145-9. pdf

Foe IT, Child MA, Majmudar JD, Krishnamurthy S, van der Linden WA, Ward GE, Martin BR, Bogyo M. Global Analysis of Palmitoylated Proteins in Toxoplasma gondii. Cell Host Microbe. 2016 18(4):501-11 pdf


2015

Oresic Bender K, Garland M, Ferreyra JA, Hryckowian AJ, Child MA, Puri AW, Solow-Cordero DE, Higgenbottom SK, Segal E, Banaei N, Shen A, Sonnenburg JL, Bogyo M. A small-molecule antivirulance agent for treating Clostridium difficile infection. Science Translational Medicine. 2015 7(306):306ra148. pdf

Staniec D, Ksiazek M, Thøgersen IB, Enghild JJ, Sroka A, Bryzek D, Bogyo M, Abrahamson M, Potempa J.Calcium Regulates the Activity and Structural Stability of Tpr, a Bacterial Calpain-like Peptidas. J Biol Chem. 2015 290(45):27248-60.

Hou L, Cooley J, Swanson R, Ong PC, Pike RN, Bogyo M, Olson ST, Remold-O'Donnell E. The protease cathepsin L regulates Th17 cell differentiation J Autoimmun. 2015 65:56-63. pdf

Edgington-Mitchell LE, Rautela J, Duivenvoorden HM, Jayatilleke KM, van der Linden WA, Verdoes M, Bogyo M, Parker BS. Cysteine cathepsin activity suppresses osteoclastogenesis of myeloid-derived suppressor cells in breast cancer. Oncotarget. 2015 6(29):27008-22. pdf

van der Linden WA, Segal E, Child MA, Byzia A, Drag M, Bogyo M. Design and Synthesis of Activity-Based Probes and Inhibitors for Bleomycin Hydrolase. Chem Biol. 2015 Aug 20;22(8):995-1001. pdf

Orlowski GM, Colbert JD, Sharma S, Bogyo M, Robertson SA, Rock KL. Multiple Cathepsins Promote Pro-IL-1β Synthesis and NLRP3-Mediated IL-1β Activation. J Immunol. 2015 Aug 15;195(4):1685-97. pdf

Lu H, Chandrasekar B, Oeljeklaus J, Misas-Villamil JC, Wang Z, Shindo T, Bogyo M, Kaiser M, van der Hoorn RA. Subfamily-specific Fluorescent Probes for Cys proteases Display Dynamic Protease Activities During Seed Germination. Plant Physiol. 2015 Jun 5. pii: pp.114.254466. [Epub ahead of print] pdf

Ofori LO, Withana NP, Prestwood TR, Verdoes M, Brady JJ, Winslow MM, Sorger J, Bogyo M. Design of Protease Activated Optical Contrast Agents That Exploit a Latent Lysosomotropic Effect for Use in Fluorescence-Guided Surgery. ACS Chem Biol. 2015 10(9):1977-88. pdf

Gangoda L, Keerthikumar S, Fonseka P, Edgington LE, Ang CS, Ozcitti C, Bogyo M, Parker BS, Mathivanan S. Inhibition of cathepsin proteases attenuates migration and sensitizes aggressive N-Myc amplified human neuroblastoma cells to doxorubicin. Oncotarget. 2015 6(13):11175-90. pdf

Oresic Bender K, Ofori L, van der Linden WA, Mock ED, Datta GK, Chowdhury S, Li H, Segal E, Sanchez Lopez M, Ellman JA, Figdor CG, Bogyo M, Verdoes M. Design of a highly selective quenched activity-based probe and its application in dual color imaging studies of cathepsin S activity localization. J Am Chem Soc. 2015 137(14):4771-7. pdf

Segal E, Prestwood TR, van der Linden WA, Carmi Y, Bhattacharya N, Withana N, Verdoes M, Habtezion A, Engleman EG, Bogyo M. Detection of intestinal cancer by local, topical application of a quenched fluorescence probe for cysteine cathepsins. Chem Biol.2015 22(1):148-58. doi: 10.1016/j.chembiol.2014.11.008. pdf

Hachmann J, Edgington-Mitchell LE, Poreba M, Sanman LE, Drag M, Bogyo M, Salvesen GS. Probes to monitor activity of the paracaspase MALT1. Chem Biol. 2015 Jan 22;22(1):139-47. doi: 10.1016/j.chembiol.2014.11.011. pdf


2014

Li H, Tsu C, Blackburn C, Li G, Hales P, Dick L, Bogyo M. Identification of potent and selective non-covalent inhibitors of the Plasmodium falciparum proteasome. J Am Chem Soc. 2014 Oct 1;136(39):13562-5. doi: 10.1021/ja507692y. pdf

Godinat A, Budin G, Morales AR, Park HM, Sanman LE, Bogyo M, Yu A, Stahl A, Dubikovskaya EA.A biocompatible "split luciferin" reaction and its application for non-invasive bioluminescent imaging of protease activity in living animals. Curr Protoc Chem Biol. 2014 Sep 9;6(3):169-89. doi: 10.1002/9780470559277.ch140047. pdf

Gangoda L, Doerflinger M, Srivastava R, Narayan N, Edgington LE, Orian J, Hawkins C, O'Reilly LA, Gu H, Bogyo M, Ekert P, Strasser A, Puthalakath H. Loss of Prkar1a leads to Bcl-2 family protein induction and cachexia in mice. Cell Death Differ. 2014 Nov;21(11):1815-24. doi: 10.1038/cdd.2014.98. pdf

Li H, van der Linden WA, Verdoes M, Florea BI, McAllister FE, Govindaswamy K, Elias JE, Bhanot P, Overkleeft HS, Bogyo M. Assessing subunit dependency of the Plasmodium proteasome using small molecule inhibitors and active site probes. ACS Chem Biol. 2014 Aug 15;9(8):1869-76. pdf

Sanman LE, Bogyo M. Activity-based profiling of proteases. Annu Rev Biochem. 2014;83:249-73. pdf

Haedke UR, Frommel SC, Hansen F, Hahne H, Kuster B, Bogyo M, Verhelst SH. Phosphoramidates as novel activity-based probes for serine proteases. Chembiochem. 2014 May 26;15(8):1106-10. pdf

Cattaruzza F, Amadesi S, Carlsson JF, Murphy JE, Lyo V, Kirkwood K, Cottrell GS, Bogyo M, Knecht W, Bunnett NW. Serine proteases and protease-activated receptor 2 mediate the proinflammatory and algesic actions of diverse stimulants. Br J Pharmacol. 2014 Aug;171(16):3814-26. pdf

Walker E, Gopalakrishnan R, Bogyo M, Basilion JP. Microscopic Detection of Quenched Activity-Based Optical Imaging Probes Using an Antibody Detection System: Localizing Protease Activity. Mol Imaging Biol. 2014 Apr 5. [Epub ahead of print] pdf

Razorenova OV, Castellini L, Colavitti R, Edgington LE, Nicolau M, Huang X, Bedogni B, Mills EM, Bogyo M, Giaccia AJ. The apoptosis repressor with a CARD domain (ARC) gene is a direct hypoxia-inducible factor 1 target gene and promotes survival and proliferation of VHL-deficient renal cancer cells. Mol Cell Biol. 2014 Feb;34(4):739-51. pdf


2013

Rothberg JM, Bailey KM, Wojtkowiak JW, Ben-Nun Y, Bogyo M, Weber E, Moin K, Blum G, Mattingly RR, Gillies RJ, Sloane BF. Acid-mediated tumor proteolysis: contribution of cysteine cathepsins. Neoplasia. 2013 Oct;15(10):1125-37. pdf

Deu E, Chen IT, Lauterwasser EM, Valderramos J, Li H, Edgington LE, Renslo AR, Bogyo M. Ferrous iron-dependent drug delivery enables controlled and selective release of therapeutic agents in vivo. Proc Natl Acad Sci U S A. 2013 Nov 5;110(45):18244-9. pdf

Ruffell B, Affara NI, Cottone L, Junankar S, Johansson M, DeNardo DG, Korets L, Reinheckel T, Sloane BF, Bogyo M, Coussens LM.Cathepsin C is a tissue-specific regulator of squamous carcinogenesis. Genes Dev. 2013 27(19):2086-98. pdf

Verdoes M, Oresic Bender K, Segal E, van der Linden WA, Syed S, Withana NP, Sanman LE, Bogyo M. An improved quenched fluorescent probe for imaging of cysteine cathepsin activity. J Am Chem Soc. 2013 135(39):14726-30. pdf

Puri AW, Bogyo M. Applications of Small Molecule Probes in Dissecting Mechanisms of Bacterial Virulence and Host Responses. Biochemistry. 2013 52(35):5985-96. pdf

Child MA, Hall CI, Beck JR, Ofori LO, Albrow VE, Garland M, Bowyer PW, Bradley PJ, Powers JC, Boothroyd JC, Weerapana E, Bogyo M. Small-molecule inhibition of a depalmitoylase enhances Toxoplasma host-cell invasion. Nat Chem Biol. 2013 9(10):651-6. pdf

Tanaka TQ, Deu E, Molina-Cruz A, Ashburne MF, Ali O, Suri A, Kortagere S, Bogyo M, Williamson KC.Plasmodium dipeptidyl aminopeptidases as malaria transmission blocking drug targets. Antimicrob Agents Chemother. 2013 Oct;57(10):4645-52. pdf

Gloeckl S, Ong VA, Patel P, Tyndall JD, Timms P, Beagley KW, Allan JA, Armitage CW, Turnbull L, Whitchurch CB, Merdanovic M, Ehrmann M, Powers JC, Oleksyszyn J, Verdoes M, Bogyo M, Huston WM. Identification of a serine protease inhibitor which causes inclusion vacuole reduction and is lethal to Chlamydia trachomatis. Mol Microbiol. 2013 Aug;89(4):676-89. pdf

Xiao J, Broz P, Puri AW, Deu E, Morell M, Monack DM, Bogyo M. coupled protein and probe engineering approach for selective inhibition and activity-based probe labeling of the caspases. J Am Chem Soc. 2013 Jun 19;135(24):9130-8. pdf

Morell M, Nguyen Duc T, Willis AL, Syed S, Lee J, Deu E, Deng Y, Xiao J, Turk BE, Jessen JR, Weiss SJ, Bogyo M. Coupling protein engineering with probe design to inhibit and image matrix metalloproteinases with controlled specificity. J Am Chem Soc. 2013 Jun 19;135(24):9139-48. pdf

Godinat A, Park HM, Miller SC, Cheng K, Hanahan D, Sanman LE, Bogyo M, Yu A, Nikitin GF, Stahl A, Dubikovskaya EA. A Biocompatible in Vivo Ligation Reaction and Its Application for Noninvasive Bioluminescent Imaging of Protease Activity in Living Mice. ACS Chem Biol. 2013 May 17;8(5):987-99. pdf

Edgington LE, Bogyo M. In vivo imaging and biochemical characterization of protease function using fluorescent activity-based probes. Curr Protoc Chem Biol. 2013 Mar 1;5(1):25-44.

Lu H, Wang Z, Shabab M, Oeljeklaus J, Verhelst SH, Kaschani F, Kaiser M, Bogyo M, van der Hoorn RA. A substrate-inspired probe monitors translocation, activation, and subcellular targeting of bacterial type III effector protease AvrPphB. Chem Biol. 2013 Feb 21;20(2):168-76. pdf

Bogyo M, Rudd PM. New technologies and their impact on 'omics' research.Curr Opin Chem Biol. 2013 Feb;17(1):1-3. pdf

Lee J, Bogyo M. Target deconvolution techniques in modern phenotypic profiling. Curr Opin Chem Biol. 2013 Feb;17(1):118-26. pdf

Misas-Villamil JC, Toenges G, Kolodziejek I, Sadaghiani AM, Kaschani F, Colby T, Bogyo M, van der Hoorn RA. Activity profiling of vacuolar processing enzymes reveals a role for VPE during oomycete infection. Plant J. 2013 Feb;73(4):689-700. pdf

Edgington LE, Verdoes M, Ortega A, Withana NP, Lee J, Syed S, Bachmann MH, Blum G, Bogyo M. Functional imaging of legumain in cancer using a new quenched activity-based probe. J Am Chem Soc. 2013 Jan 9;135(1):174-82. pdf


2012

Mullins SR, Sameni M, Blum G, Bogyo M, Sloane BF, Moin K.Three-dimensional cultures modeling premalignant progression of human breast epithelial cells: role of cysteine cathepsins. Biol Chem. 2012 Dec;393(12):1405-16. pdf

Stolze SC, Deu E, Kaschani F, Li N, Florea BI, Richau KH, Colby T, van der Hoorn RA, Overkleeft HS, Bogyo M, Kaiser M. The antimalarial natural product symplostatin 4 is a nanomolar inhibitor of the food vacuole falcipains. Chem Biol. 2012 Dec 21;19(12):1546-55. pdf

Li H, Ponder EL, Verdoes M, Asbjornsdottir KH, Deu E, Edgington LE, Lee JT, Kirk CJ, Demo SD, Williamson KC, Bogyo M. Validation of the proteasome as a therapeutic target in Plasmodium using an epoxyketone inhibitor with parasite-specific toxicity. Chem Biol. 2012 Dec 21;19(12):1535-45. pdf

Sztukowska M, Veillard F, Potempa B, Bogyo M, Enghild JJ, Thogersen IB, Nguyen KA, Potempa J. Disruption of gingipain oligomerization into non-covalent cell-surface attached complexes. Biol Chem. 2012 Sep;393(9):971-7. pdf

Salvesen GS, Bogyo M. Highlight: the universe of proteolytic networks and mechanisms. Biol Chem. 2012 Sep;393(9):841. pdf

Lyo V, Cattaruzza F, Kim TN, Walker AW, Paulick M, Cox D, Cloyd J, Buxbaum J, Ostroff J, Bogyo M, Grady EF, Bunnett NW, Kirkwood KS. Active cathepsins B, L, and S in murine and human pancreatitis. Am J Physiol Gastrointest Liver Physiol. 2012 Oct 15;303(8):G894-903. pdf

Goussetis DJ, Gounaris E, Wu EJ, Vakana E, Sharma B, Bogyo M, Altman JK, Platanias LC. Autophagic degradation of the BCR-ABL oncoprotein and generation of antileukemic responses by arsenic trioxide. Blood. 2012 Oct 25;120(17):3555-62. pdf

Puri AW, Broz P, Shen A, Monack DM, Bogyo M. Caspase-1 activity is required to bypass macrophage apoptosis upon Salmonella infection. Nature Chemical Biology. 2012; 8(9): 745-7. pdf

Verdoes M, Edgington LE, Scheeren FA, Leyva M, Blum G, Weiskopf K, Bachmann MH, Ellman JA, Bogyo M. A nonpeptidic cathepsin s activity-based probe for noninvasive optical imaging of tumor-associated macrophages. Chemistry & Biology. 2012; 19(5):619-28. pdf

Cutter JL, Cohen NT, Wang J, Sloan AE, Cohen AR, Panneerselvam A, Schluchter M, Blum G, Bogyo M, Basilion JP. Topical application of activity-based probes for visualization of brain tumor tissue. PLoS One. 2012;7(3):e33060. Epub 2012 Mar 13. pdf

Richau KH, Kaschani F, Verdoes M, Pansuriya TC, Niessen S, Stüber K, Colby T, Overkleeft HS, Bogyo M, Van der Hoorn RA. Subclassification and biochemical analysis of plant papain-like cysteine proteases displays subfamily-specific characteristics. Plant Physiol. 2012 Apr;158(4):1583-99. pdf

Edgington LE, van Raam BJ, Verdoes M, Wierschem C, Salvesen GS, Bogyo, M. An optimized activity-based probe for the study of caspase-6 activation. Chemistry & Biology. 2012; 19(3): 340-52 pdf

Fritz HM, Bowyer PW, Bogyo M, Conrad PA, Boothroyd JC. Proteomic analysis of fractionated toxoplasma oocysts reveals clues to their environmental resistance. PLoS One. 2012;7(1):e29955. pdf

Withana NP, Blum G, Sameni M, Slaney C, Anbalagan A, Olive MB, Bidwell BN, Edgington L, Wang L, Moin K, Sloane BF, Anderson RL, Bogyo MS, Parker BS. Cathepsin B inhibition limits bone metastasis in breast cancer. Cancer Res. 2012 Jan 19. [Epub ahead of print]. pdf

Ferreira KS, Kreutz C, Macnelly S, Neubert K, Haber A, Bogyo M, Timmer J, Borner C. Caspase-3 feeds back on caspase-8, Bid and XIAP in type I Fas signaling in primary mouse hepatocytes. Apoptosis. 2012 Jan 13. [Epub ahead of print]. pdf

Lee J, Bogyo M. Synthesis and evaluation of aza-peptidyl inhibitors of the lysosomal asparaginyl endopeptidase, legumain.Bioorg Med Chem Lett. 2012 Feb 1;22(3):1340-3. pdf

Deu E, Verdoes M, Bogyo M. New approaches for dissecting protease functions to improve probe development and drug discovery. Nat Struct Mol Biol. 2012 Jan 5;19(1):9-16. pdf

Li H, Child MA, Bogyo M. Proteases as regulators of pathogenesis: Examples from the apicomplexa. Biochim Biophys Acta. 2012 1824(1):177-85 pdf

Kalinska M, Kantyka T, Greenbaum DC, Larsen KS, W?adyka B, Jabaiah A, Bogyo M, Daugherty PS, Wysocka M, Jaros M, Lesner A, Rolka K, Schaschke N, Stennicke H, Dubin A, Potempa J, Dubin G. Substrate specificity of Staphylococcus aureus cysteine proteases - Staphopains A, B and C. Biochimie. 2012 94(2):318-27. pdf


2011

Edgington LE and Bogyo M. (2011) Applications for Activity-based Probes in Drug Discovery. Royal Society of Chemistry. Ed. Bunage M. P. 33-63. pdf

Ren G, Blum G, Verdoes M, Liu H, Syed S, Edgington LE, Gheysens O, Miao Z, Jiang H, Gambhir SS, Bogyo M, Cheng Z. Non-invasive imaging of cysteine cathepsin activity in solid tumors using a 64Cu-labeled activity-based probe. PLoS One. 2011;6(11):e28029. pdf

Edgington LE, Verdoes M, Bogyo M. Functional imaging of proteases: recent advances in the design and application of substrate-based and activity-based probes. Curr Opin Chem Biol. 2011 Dec;15(6):798-805. pdf

Li J, Hsu HC, Yang P, Wu Q, Li H, Edgington LE, Bogyo M, Kimberly RP, Mountz JD. Treatment of arthritis by macrophage depletion and immunomodulation: Testing an apoptosis-mediated therapy in a humanized death receptor mouse model. Arthritis Rheum. 2011 Oct 17. doi: 10.1002/art.33423. [Epub ahead of print]. pdf

Tedelind S, Jordans S, Resemann H, Blum G, Bogyo M, Führer D, Brix K. Cathepsin B trafficking in thyroid carcinoma cells. Thyroid Res. 2011 Aug 3;4 Suppl 1:S2. pdf

Mikhaylov G, Mikac U, Magaeva AA, Itin VI, Naiden EP, Psakhye I, Babes L, Reinheckel T, Peters C, Zeiser R, Bogyo M, Turk V, Psakhye SG, Turk B, Vasiljeva O. Ferri-liposomes as an MRI-visible drug-delivery system for targeting tumours and their microenvironment. Nat Nanotechnol. 2011 6(9):594-602. pdf

Cattaruzza F, Lyo V, Jones E, Pham D, Hawkins J, Kirkwood K, Valdez-Morales E, Ibeakanma C, Vanner SJ, Bogyo M, Bunnett NW. Cathepsin S is activated during colitis and causes visceral hyperalgesia by a PAR2-dependent mechanism in mice. Gastroenterology. 2011 141(5):1864-74. pdf

Hall CI, Reese ML, Weerapana E, Child MA, Bowyer PW, Albrow VE, Haraldsen JD, Phillips MR, Sandoval ED, Ward GE, Cravatt BF, Boothroyd JC, Bogyo M. Chemical genetic screen identifies Toxoplasma DJ-1 as a regulator of parasite secretion, attachment, and invasion. Proc Natl Acad Sci U S A. 2011 108(26):10568-73. pdf

Boutté AM, Friedman DB, Bogyo M, Min Y, Yang L, Lin PC. Identification of a myeloid-derived suppressor cell cystatin-like protein that inhibits metastasis. FASEB J. 2011 25(8):2626-37 pdf

Ponder EL, Albrow VE, Leader BA, Békés M, Mikolajczyk J, Fonovi? UP, Shen A, Drag M, Xiao J, Deu E, Campbell AJ, Powers JC, Salvesen GS, Bogyo M. Functional Characterization of a SUMO Deconjugating Protease of Plasmodium falciparum Using Newly Identified Small Molecule Inhibitors. Chem Biol. 2011 18:711-721. pdf

Albrow VE, Ponder EL, Fasci D, Békés M, Deu E, Salvesen GS, Bogyo M. Development of Small Molecule Inhibitors and Probes of Human SUMO Deconjugating Proteases. Chem Biol. 2011 18:722-732. pdf

Ewald SE, Engel A, Lee J, Wang M, Bogyo M, Barton GM. Nucleic acid recognition by Toll-like receptors is coupled to stepwise processing by cathepsins and asparagine endopeptidase. J Exp Med. 2011 208:643-51. pdf

Paulick MG, Bogyo M. Development of Activity-Based Probes for Cathepsin X. ACS Chem Biol. 2011 6:563-72. pdf

Mahajan SS, Deu E, Lauterwasser EM, Leyva MJ, Ellman JA, Bogyo M, Renslo AR. A fragmenting hybrid approach for targeted delivery of multiple therapeutic agents to the malaria parasite. ChemMedChem. 2011 6:415-9. pdf

Shen A, Lupardus PJ, Gersch MM, Puri AW, Albrow VE, Garcia KC Bogyo M. Defining an allosteric circuit in the cysteine protease domain of Clostridium difficile toxins. Nature Struc. Mol. Bio. 2011. 18: 364-7. pdf

Wang F, Krai P, Deu E, Bibb B, Lauritzen C, Pedersen J, Bogyo M, Klemba M. Biochemical characterization of Plasmodium falciparum dipeptidyl aminopeptidase 1. Mol Biochem Parasitol. 2011 175:10-20. pdf

Bowyer PW, Simon GM, Cravatt BF, Bogyo M. Global profiling of proteolysis during rupture of P. falciparum from the host erythrocyte. Mol Cell Proteomics. 2011 10(5):M110.001636. pdf


2010

Puri AW, Lupardus PJ, Deu E, Albrow VE, Garcia KC, Bogyo M, Shen A. Rational design of inhibitors and activity-based probes targeting Clostridium difficile virulence factor TcdB. Chem Biol. 2010 17: 1201-1211. pdf

Deu E, Leyva MJ, Albrow VE, Rice MJ, Ellman JA, Bogyo M. Functional studies of Plasmodium falciparum dipeptidyl aminopeptidase I using small molecule inhibitors and active site probes. Chem Biol. 2010 17:808-19. pdf

Deu E, Yang Z, Wang F, Klemba M, Bogyo M. Use of activity-based probes to develop high throughput screening assays that can be performed in complex cell extracts. PLoS One. 2010 5(8). pii: e11985. pdf

Tedelind S, Poliakova K, Valeta A, Hunegnaw R, Yemanaberhan EL, Heldin NE, Kurebayashi J, Weber E, Kopitar-Jerala N, Turk B, Bogyo M, Brix K. Nuclear cysteine cathepsin variants in thyroid carcinoma cells.Biol Chem. 2010 391:923-35. pdf

Staudt ND, Aicher WK, Kalbacher H, Stevanovic S, Carmona AK, Bogyo M, Klein G. Cathepsin X is secreted by human osteoblasts, digests CXCL-12 and impairs adhesion of hematopoietic stem and progenitor cells to osteoblasts. Haematologica. 2010 95(9):1452-60. pdf

Konjar S, Yin F, Bogyo M, Turk B, Kopitar-Jerala N.Increased nucleolar localization of SpiA3G in classically but not alternatively activated macrophages. FEBS Lett. 2010 Mar 23. [Epub ahead of print]. pdf

Bogyo M. Finding enzymes that are actively involved in cancer. Proc Natl Acad Sci U S A. 2010 Feb 9;107(6):2379-80. pdf

Lee J, Bogyo M. Development of near-infrared fluorophore (NIRF)-labeled activity-based probes for in vivo imaging of legumain. ACS Chem Biol. 2010 19:233-43. pdf

Drag M, Bogyo M, Ellman JA, Salvesen GS. Aminopeptidase fingerprints. An integrated approach for identification of good substrates and optimal inhibitors. J Biol Chem. (2010) 285:3310-8. pdf


2009

Ravindran S, Lodoen MB, Verhelst SH, Bogyo M, Boothroyd JC. 4-bromophenacyl bromide specifically inhibits rhoptry secretion during Toxoplasma invasion. PLoS One. (2009) Dec 2;4(12):e8143. pdf

Shen A, Lupardus PJ, Morell M, Ponder EL, Sadaghiani AM, Garcia KC, Bogyo M. Simplified, enhanced protein purification using an inducible, autoprocessing enzyme tag. PLoS One. (2009) Dec 2;4(12):e8119.pdf

Horn M, Nussbaumerová M, Sanda M, Kovárová Z, Srba J, Franta Z, Sojka D, Bogyo M, Caffrey CR, Kopácek P, Mares M. Hemoglobin digestion in blood-feeding ticks: mapping a multipeptidase pathway by functional proteomics. Chem Biol. (2009) 16:1053-63. pdf

Zhang J, Fonovic M, Suyama K, Bogyo M, Scott MP. Rab35 controls actin bundling by recruiting fascin as an effector protein. Science. (2009) 325:1250-4. pdf

Blum G, Weimer RM, Edgington LE, Adams W, Bogyo M. Comparative assessment of substrates and activity based probes as tools for non-invasive optical imaging of cysteine protease activity. PLoS One(2009) 4(7): e6374 pdf

Puri AW, Bogyo M. Using Small Molecules to Dissect Mechanisms of Microbial Pathogenesis. ACS Chem. Biol. (2009) July 16. [Epub ahead of print]. pdf about the author

Lee JT, Chen DY, Yang Z, Ramos AD, Hsieh JJD, Bogyo M. Design, synthesis, and evaluation of Taspase1 inhibitors. Bioorg. Med. Chem. Let. (2009) 19:5086-5090 pdf

Edgington LE, Berger AB, Blum G, Albrow VE, Paulick MG, Lineberry N, Bogyo M. Noinvasive optical imaging of apoptosis by caspase-targeted activity based probes. Nat Med (2009) 15:967-73 pdf Stanford Press Release

Larson ET, Parussini F, Huynh MH, Giebel JD, Kelley AM, Zhang L, Bogyo M, Merritt EA, Carruthers VB. Toxoplasma gondii cathepsin l is the primary target of the invasion inhibitory compound LHVS. J. Biol. Chem. (2009) July 17. [Epub ahead of print]. pdf

Shen A, Lupardus PJ, Albrow VE, Guzzetta A, Powers JC, Garcia KC, Bogyo M. Mechanistic and structural insights into the proteolytic activation of Vibrio cholerae MARTX toxin. Nat Chem Biol. (2009) 5:469-478. pdf

Chang SH, Kanasaki K, Gocheva V, Blum G, Harper J, Moses MA, Shih SC, Nagy JA, Joyce J, Bogyo M, Kalluri R, Dvorak HF. VEGF-A induces angiogenesis by perturbing the cathepsin-cysteine protease inhibitor balance in venules, causing basement membrane degradation and mother vessel formation. Cancer Res. (2009) 69:4537-44. pdf

Beckham SA, Piedrafita D, Phillips CI, Samarawickrema N, Law RH, Smooker PM, Quinsey NS, Irving JA, Greenwood D, Verhelst SH, Bogyo M, Turk B, Coetzer TH, Wijeyewickrema LC, Spithill TW, Pike RN. A major cathepsin B protease from the liver fluke Fasciola hepatica has atypical active site features and a potential role in the digestive tract of newly excysted juvenile parasites. Int J Biochem Cell Biol. (2009) 41:1601-12. pdf

Barbero S, Mielgo A, Torres V, Teitz T, Shields DJ, Mikolon D, Bogyo M, Barilà D, Lahti JM, Schlaepfer D, Stupack DG. Caspase-8 association with the focal adhesion complex promotes tumor cell migration and metastasis. Cancer Res. (2009) 69:3755-63. pdf

Cavallo-Medved D, Rudy D, Blum G, Bogyo M, Caglic D, Sloane BF. Live-cell imaging demonstrates extracellular matrix degradation in association with active cathepsin B in caveolae of endothelial cells during tube formation. Exp Cell Res. (2009) 315:1234-46. pdf

Obermajer N, Jevnikar Z, Doljak B, Sadaghiani AM, Bogyo M, Kos J. Cathepsin X-mediated beta2 integrin activation results in nanotube outgrowth. Cell Mol Life Sci. (2009) 66:1126-34. pdf

Pungercar JR, Caglic D, Sajid M, Dolinar M, Vasiljeva O, Pozgan U, Turk D, Bogyo M, Turk V, Turk B. Autocatalytic processing of procathepsin B is triggered by proenzyme activity. FEBS J. (2009) 276:660-8. pdf

Liz MA, Fleming CE, Nunes AF, Almeida MR, Mar FM, Choe Y, Craik CS, Powers JC, Bogyo M, Sousa MM. Substrate specificity of transthyretin: identification of natural substrates in the nervous system. Biochem J. (2009) Biochem J. 2009 Apr 15;419(2):467-74. pdf

Bogyo M. Metabolomics cuts to the chase to chase the cuts. Nat Chem Biol. (2009) 5:5-6. pdf


2008

Fonovic M, Bogyo M. Activity-based probes as a tool for functional proteomic analysis of proteases. Expert Rev Proteomics. (2008) 5:721-30. pdf

Lupardus PJ, Shen A, Bogyo M, Garcia KC.Small molecule-induced allosteric activation of the Vibrio cholerae RTX cysteine protease domain. Science. (2008) 322:265-8. pdf Sup. Mat.

Shen A, Bogyo M. Friend or Foe? Turning a Host Defense Protein Into a Pathogen's Accomplice. Chem Biol.(2008) 15:879-80. pdf

Kaschani F, Verhelst SH, van Swieten PF, Verdoes M, Wong CS, Wang Z, Kaiser M, Overkleeft HS, Bogyo M, van der Hoorn RA. Minitags for small molecules: detecting targets of reactive small molecules in living plant tissues using 'click-chemistry' Plant J. (2008) 57:373-85. pdf

Schurigt U, Sevenich L, Vannier C, Gajda M, Schwinde A, Werner F, Stahl A, von Elverfeldt D, Becker AK, Bogyo M, Peters C, Reinheckel T. Trial of the cysteine cathepsin inhibitor JPM-OEt on early and advanced mammary cancer stages in the MMTV-PyMT-transgenic mouse model. Biol Chem. (2008) 389:1067-74. pdf

Obermajer N, Svajger U, Bogyo M, Jeras M, Kos J. Maturation of dendritic cells depends on proteolytic cleavage by cathepsin X. J Leukoc Biol. (2008) 84:1306-14. pdf

Jevnikar Z, Obermajer N, Bogyo M, Kos J. The role of cathepsin X in the migration and invasiveness of T lymphocytes. J Cell Sci. (2008) 121:2652-61. pdf

Yang Z, Fonovic M, Verhelst SH, Blum G and Bogyo M. Evaluation of alpha,beta-unsaturated ketone-based probes for papain-family cysteine proteases. Bioorg Med Chem. (2008) Mar 2. [Epub ahead of print]. pdf.

Paulick MG, Bogyo M. Application of activity-based probes to the study of enzymes involved in cancer progression. Curr Opin Genet Dev. (2008) 18:97-106. pdf.

Arastu-Kapur S, Ponder EL, Fonovi? UP, Yeoh S, Yuan F, Fonovic M, Grainger M, Phillips CI, Powers JC, and Bogyo M. Identification of proteases that regulate erythrocyte rupture by the malaria parasite Plasmodium falciparum. Nature Chemical Biology (2008) 4:203-213. pdf. News and Views pdf


2007
Ponder EL and Bogyo M. Ubiquitin-like modifiers and their deconjugating enzymes in medically important parasitic protozoa.Eukaryot Cell. (2007) 6:1943-1952. pdf.

Goulet B, Sansregret L, Leduy L, Bogyo M, Weber E, Chauhan SS, Nepveu A. Increased expression and activity of nuclear cathepsin L in cancer cells suggests a novel mechanism of cell transformation. Mol Cancer Res. (2007) 5:899-907. pdf.

Blum, G., von Degenfeld, G., Merchant, M.J., Blau, H.M., and Bogyo, M. Noinvasive optical imaging of cysteine protease activity using fluorescently quenched activity-based probes. (2007) Nature Chemical Biology. 3: 668-677. pdf.

Fonovic M, Verhelst SH, Sorum MT, Bogyo M. Proteomic evaluation of chemically cleavable activity based probes. (2007) Mol Cell Proteomics. 6: 1761-1770. pdf

Bell-McGuinn KM, Garfall AL, Bogyo M, Hanahan D, Joyce JA. Inhibition of cysteine cathepsin protease activity enhances chemotherapy regimens by decreasing tumor growth and invasiveness in a mouse model of multistage cancer. (2007) Cancer Res. 67:7378-85. pdf

Burster T, Giffon T, Dahl ME, Bjorck P, Bogyo M, Weber E, Mahmood K, Lewis DB, Mellins ED. Influenza A virus elevates active cathepsin B in primary murine DC. (2007) Int Immunol. 19:645-55. pdf

Rose PP, Bogyo M, Moses AV, Fruh K. Insulin-like growth factor II receptor-mediated intracellular retention of cathepsin B is essential for transformation of endothelial cells by Kaposi's sarcoma-associated herpesvirus. (2007) J Virol. 81:8050-62. pdf

Sadaghiani, A.M.,.Verhelst, S.H.L., Gocheva, V., Hill, K., Majerova, E., Stinson, S. Joyce, J.A. and Bogyo, M. Design, synthesis and evaluation of in vivo potency and selectivity of epoxysuccinyl-based inhibitors of papain family cysteine proteases. (2007) Chem Biol 14:499-511. pdf

Sojka D, Hajdusek O, Dvorak J, Sajid M, Franta Z, Schneider EL, Craik CS, Vancova M, Buresova V, Bogyo M, Sexton KB, McKerrow JH, Caffrey CR, Kopacek P. IrAE - An asparaginyl endopeptidase (legumain) in the gut of the hard tick Ixodes ricinus. (2007) Int J Parasitol. 37:713-724.

Cuerrier D, Moldoveanu T, Campbell RL, Kelly J, Yoruk B, Verhelst SH, Greenbaum D, Bogyo M, Davies PL. Development of calpain-specific inactivators by screening of positional-scanning epoxide libraries. (2007) J Biol Chem. 282:9600-96011. pdf

Bogyo, M and Cravatt BF. Genomics and proteomics From genes to function: advances in applications of chemical and systems biology. (2007) Curr Opin Chem Biol. 11:1-3. pdf

Teo CF, Zhou XW, Bogyo, M, Carruthers VB. Cysteine protease inhibitors block Toxoplasma gondii microneme secretion and cell invasion. (2007) Antimicrob Agents Chemother. 51, 679-88. pdf

Verhelst, S.H.L., Fonovic, M. and Bogyo, M. A Mild Chemically Cleavable Linker System for Functional Proteomic Applications. (2007) Angew. Chemie. 46:1284-1286. pdf

Fonovic, M. and Bogyo, M. Activity Based Probes for Proteases: Applications to Biomarker Discovery, Molecular Imaging and Drug Screening. (2007) Current Pharmaceutical Design. 13:253-261 pdf

Sadaghiani, A.M., Verhelst, S.H.L., and Bogyo, M.Tagging and Detection Strategies for Activity-Based Proteomics. (2007) Cur Opin Chem. Bio. 11:20-28. pdf

Sexton, K.B., Witte, M.D., Blum, G., and Bogyo, M. Design of cell permeable, fluorescent activity based probes of the lysosomal cysteine protease asparaginyl endopeptidase (AEP)/legumain. (2007) Bioorg. Med. Chem. Let. 17:649-653. pdf

Sexton, K.B., Kato, D., Berger, A.B., Fonovic, M., Verhelst, S.H.L. and Bogyo, M. Specificity if aza-peptide electrophile activity based probes of caspases. (2007) Cell Death Differ 14:727-732. pdf


2006
Berger, A.B., Sexton, K.B., and Bogyo, M. Commonly used caspase inhibitors designed based on substrate specificity profiles lack selectivity. (2006) Cell Research. 16:961-963. pdf

Sadaghiani, A. M.,Verhelst, S. H. L. and Bogyo, M. Solid-Phase Methods for the Preparation of Epoxysuccinate-Based Inhibitors of Cysteine Proteases. (2006) J. Comb. Chem. 8: 802-804. pdf Sup Mat

Pandey, K.C., Singh, N., Arastu-Kapur, S., Bogyo, M. and Rosenthal P.J. Falstatin, a Cysteine Protease Inhibitor of Plasmodium falciparum, Facilitates Erythrocyte Invasion. (2006) PloS Pathogens. 2: e117 pdf

Denault, J.-B., Bekes, M., Scott, F.L., Sexton, K.B., Bogyo, M. and Salvesen, G.S., Engineered hybrid dimers: tracking the activation pathway of caspase-7. (2006) Molecular Cell. 23:523-533. pdf

Berger, A.B., Witte, M.D., Denault, J.-B., Sadaghiani, A.M., Sexton, K.B., Salvesen, G.S. and Bogyo, M. Identification of early intermediates of caspase activation using selective inhibitors and activity-based probes . (2006) Molecular Cell. 23:509-521. pdf 

Vasiljeva, O., Papazoglou, A., Kruger, A., Brodoefel, H., Korovin, M., Deussing, J., Augustin, N., Nielsen, B.S., Almholt, K., Bogyo, M., Peters, C. and Reinheckel, T. Tumor cell-derived and macrophage-derived cathepsin B promotes progression and lung metastasis of mammary cancer. (2006) Cancer Res. 66:5242-5250. pdf

Yuan, F., Verhelst,  S., Blum, G., Coussens, L. and Bogyo, M. A selective activity-based probe for the papain family cysteine protease dipeptidyl peptidase I/cathepsin C. (2006) J. Am. Chem. Soc. 128:5616-5617. pdf

Verhelst, S.H.L., Witte, M.D., Arastu-KApur, S., Fonovic M., and Bogyo, M. Novel aza peptide inhibitors and active site probes of papain family cysteine proteases. (2006) ChemBioChem, epub ahead of print. pdf.

Pan Z, Jeffery DA, Chehade K, Beltman J, Clark JM, Grothaus P, Bogyo M, and Baruch A. Development of activity-based probes for trypsin-family serine proteases. (2006) Bioorg Med Chem Lett. 16:2882-2885. pdf

Choe Y., Leonetti F., Greenbaum D.C., Lecaille F., Bogyo M., Bromme D., Ellman J.A. andCraik C.S. Substrate profiling of cysteine proteases using a combinatorial peptide library identifies functionally unique specificities.(2006) J Biol Chem. 281:12824-12832. pd


2005
Kato, D., Verhelst, S.H.L., Sexton, K.B. and Bogyo, M. A General Solid Phase Method for the Preparation of Diverse Azapeptide Probes Directed Against Cysteine Proteases. (2005) Organic Letters, 7:5649-5652.pdf

Blum, G., Mullins, S.R., Keren, K., Fonovic, M., Jedeszko, C., Rice, M.J., Sloane, B.F. and Bogyo, M. Dynamic imaging of protease activity with fluorescently quenched activity-based probes. (2005) Nature Chemical Biology, 1:203-209.pdf Nature Chemical Biology website News and Views

Kato, D., Boatright, K.M., Berger, A.B., Nazif, T., Blum, G., Ryan, C., Chehade, K.A.H., Salvesen, G., and Bogyo, M. Activity based probes that target diverse cysteine protease families. (2005) Nature Chemical Biology 1:33-38. pdf Nature Chemical Biology website

Verhelst, S.H.L, and Bogyo, M. Dissecting protein function using chemical proteomic methods. (2005) QSAR in press.

Phillips, C.I., and Bogyo, M. Proteomics meets microbiology: technical advances in the global mapping of protein expression and function. (2005) Cellular Microbiology 7(8):1061-76. Abstract pdf

Verhelst S.H.L. and Bogyo, M. Solid-Phase Synthesis of Double-Headed Epoxysuccinyl Activity-Based Probes for Selective Targeting of Papain Family Cysteine Proteases. (2005) ChemBioChem 6(5):824-827. Abstract pdf

Verhelst S.H.L. and Bogyo, M. Chemical proteomics applied to target identification and drug discovery. (2005) Biotechniques 38(2):175-7. Abstract pdf

Chehade, K.A.H., Baruch, A., Verhelst, S.H.L. and Bogyo, M.An improved preparation of the activity-based probe JPM-OEt and in situ applications. (2005) Synthesis 2: 240-244. pdf

Borodovsky, A., Ovaa. H., Meester, W.J., Venanzi, E.S., Bogyo, M., Hekking B.G., Ploegh, H.L., Kessler, B.M. and Overkleeft, H.S. Small-molecule inhibitors and probes for ubiquitin- and ubiquitin-like-specific proteases. (2005) Chembiochem 6(2):287-91. Abstract pdf


2004
Berger, A.B., Vitorino, P.M., Bogyo, M. Activity-based protein profiling: applications to biomarker discovery, in vivo imaging and drug discovery. (2004) Am J Pharmacogenomics 4(6):371-81. Abstract pdf

Snipas, SJ, Wildfang E, Nazif T, Christensen L, Boatright KM, Bogyo M, Stennicke HR, Salvesen GS Characteristics of the caspase-like catalytic domain of human paracaspase. (2004) Biol Chem 385(11):1093-8. Abstract pdf

van der Hoorn RA, Leeuwenburgh MA, Bogyo M, Joosten MH, Peck SC Activity profiling of papain-like cysteine proteases in plants. (2004) Plant Physiol 135(3):1170-8. Abstract pdf

Oleksy A, Golonka E, Banbula A, Szmyd G, Moon J, Kubica M, Greenbaum D, Bogyo M, Foster TJ, Travis J, Potempa J Growth phase-dependent production of a cell wall-associated elastinolytic cysteine proteinase by Staphylococcus epidermidis. (2004) Biol Chem 385(6):525-35. Abstract pdf

Hook V, Yasothornsrikul S, Greenbaum D, Medzihradszky KF, Troutner K, Toneff T, Bundey R, Logrinova A, Reinheckel T, Peters C, Bogyo M Cathepsin L and Arg/Lys aminopeptidase: a distinct prohormone processing pathway for the biosynthesis of peptide neurotransmitters and hormones. (2004) Biol Chem 385(6):473-80. Abstract pdf

Eksi S, Czesny B, Greenbaum DC, Bogyo M, Williamson KC Targeted disruption of Plasmodium falciparum cysteine protease, falcipain 1, reduces oocyst production, not erythrocytic stage growth. (2004) Mol Microbiol 53(1):243-50. Abstract pdf

Yasuda Y, Li Z, Greenbaum D, Bogyo M, Weber E, Bromme D Cathepsin V, a novel and potent elastolytic activity expressed in activated macrophages. (2004) J Biol Chem 279(35):36761-70. Abstract pdf

Joyce JA, Baruch A, Chehade K, Meyer-Morse N, Giraudo E, Tsai FY, Greenbaum DC, Hager JH, Bogyo M, Hanahan D Cathepsin cysteine proteases are effectors of invasive growth and angiogenesis during multistage tumorigenesis. (2004) Cancer Cell 5(5):443-53. Abstract pdf

Goulet B, Baruch A, Moon NS, Poirier M, Sansregret LL, Erickson A, Bogyo M, Nepveu A A cathepsin L isoform that is devoid of a signal peptide localizes to the nucleus in S phase and processes the CDP/Cux transcription factor. (2004) Mol Cell 14(2):207-19. Abstract pdf

Medzihradszky KF, Darula Z, Perlson E, Fainzilber M, Chalkley RJ, Ball H, Greenbaum D, Bogyo M, Tyson DR, Bradshaw RA, Burlingame AL O-sulfonation of serine and threonine: mass spectrometric detection and characterization of a new posttranslational modification in diverse proteins throughout the eukaryotes. (2004) Mol Cell Proteomics 3(5):429-40. Abstract pdf

Baruch A, Jeffery DA, Bogyo M Enzyme activity--it's all about image. (2004) Trends Cell Biol 14(1):29-35. Abstract pdf

Li Z, Yasuda Y, Li W, Bogyo M, Katz N, Gordon RE, Fields GB, Bromme D Regulation of collagenase activities of human cathepsins by glycosaminoglycans. (2004) J Biol Chem 279(7):5470-9. Abstract pdf


2003
Sajid M, McKerrow JH, Hansell E, Mathieu MA, Lucas KD, Hsieh I, Greenbaum D, Bogyo M, Salter JP, Lim KC, Franklin C, Kim JH, Caffrey CR Functional expression and characterization of Schistosoma mansoni cathepsin B and its trans-activation by an endogenous asparaginyl endopeptidase. (2003) Mol Biochem Parasitol 131(1):65-75. Abstract pdf

Yasothornsrikul S, Greenbaum D, Medzihradszky KF, Toneff T, Bundey R, Miller R, Schilling B, Petermann I, Dehnert J, Logvinova A, Goldsmith P, Neveu JM, Lane WS, Gibson B, Reinheckel T, Peters C, Bogyo M, Hook V Cathepsin L in secretory vesicles functions as a prohormone-processing enzyme for production of the enkephalin peptide neurotransmitter. (2003) Proc Natl Acad Sci 100(16):9590-5. Abstract pdf

Rozman-Pungercar J, Kopitar-Jerala N, Bogyo M, Turk D, Vasiljeva O, Stefe I, Vandenabeele P, Bromme D, Puizdar V, Fonovic M, Trstenjak-Prebanda M, Dolenc I, Turk V, Turk B Inhibition of papain-like cysteine proteases and legumain by caspase-specific inhibitors: when reaction mechanism is more important than specificity. (2003) Cell Death Differ 10(8):881-8. Abstract pdf

Wang CC, Bozdech Z, Liu CL, Shipway A, Backes BJ, Harris JL, Bogyo M Biochemical analysis of the 20S proteasome of Trypanosoma brucei. (2003) J Biol Chem 278(18):15800-8. Abstract pdf

Mikolajczyk J, Boatright KM, Stennicke HR, Nazif T, Potempa J, Bogyo M, Salvesen GS Sequential autolytic processing activates the zymogen of Arg-gingipain. (2003) J Biol Chem 278(12):10458-64. Abstract pdf

Kessler B, Hong X, Petrovic J, Borodovsky A, Dantuma NP, Bogyo M, Overkleeft HS, Ploegh H, Glas R Pathways accessory to proteasomal proteolysis are less efficient in major histocompatibility complex class I antigen production. (2003) J Biol Chem 278(12):10013-21. Abstract pdf

Jeffery DA, Bogyo M Chemical proteomics and its application to drug discovery. (2003) Curr OpinBiotechnol 14(1):87-95. Abstract pdf


2002
Greenbaum DC, Baruch A, Grainger M, Bozdech Z, Medzihradszky KF, Engel J, DeRisi J, Holder AA, Bogyo M A role for the protease falcipain 1 in host cell invasion by the human malaria parasite. (2002) Science 298(5600):2002-6. Abstract pdf

Greenbaum DC, Arnold WD, Lu F, Hayrapetian L, Baruch A, Krumrine J, Toba S, Chehade K, Bromme D, Kuntz ID, Bogyo M Small molecule affinity fingerprinting. A tool for enzyme family subclassification, target identification, and inhibitor design. (2002) Chem Biol 9(10):1085-94. Abstract pdf

Groll M, Nazif T, Huber R, Bogyo M Probing structural determinants distal to the site of hydrolysis that control substrate specificity of the 20S proteasome. (2002) Chem Biol 9(5):655-62. Abstract pdf

Mathieu MA, Bogyo M, Caffrey CR, Choe Y, Lee J, Chapman H, Sajid M, Craik CS, McKerrow JH Substrate specificity of schistosome versus human legumain determined by P1-P3 peptide libraries. (2002) Mol Biochem Parasitol 121(1):99-105. Abstract pdf

Bogyo M, Wang EW Proteasome inhibitors: complex tools for a complex enzyme. (2002) Curr Top Microbiol Immunol 268:185-208. Abstract pdf

Greenbaum D, Baruch A, Hayrapetian L, Darula Z, Burlingame A, Medzihradszky KF, Bogyo M Chemical approaches for functionally probing the proteome. (2002) Mol Cell Proteomics 1(1):60-8. Abstract pdf


2001
Caffrey CR, Hansell E, Lucas KD, Brinen LS, Alvarez Hernandez A, Cheng J, Gwaltney SL 2nd, Roush WR, Stierhof YD, Bogyo M, Steverding D, McKerrow JH Active site mapping, biochemical properties and subcellular localization of rhodesain, the major cysteine protease of Trypanosoma brucei rhodesiense. (2001) Mol Biochem Parasitol 118(1):61-73. Abstract pdf

Baruch A, Greenbaum D, Levy ET, Nielsen PA, Gilula NB, Kumar NM, Bogyo M Defining a link between gap junction communication, proteolysis, and cataract formation. (2001) J Biol Chem 276(31):28999-9006. Abstract pdf

Li J, Gao X, Ortega J, Nazif T, Joss L, Bogyo M, Steven AC, Rechsteiner M Lysine 188 substitutions convert the pattern of proteasome activation by REGgamma to that of REGs alpha and beta. (2001) EMBO J 20(13):3359-69. Abstract pdf

Nazif T, Bogyo M Global analysis of proteasomal substrate specificity using positional-scanning libraries of covalent inhibitors. (2001) Proc Natl Acad Sci 98(6):2967-72. Abstract pdf


2000
Weihofen A, Lemberg MK, Ploegh HL, Bogyo M, Martoglio B Release of signal peptide fragments into the cytosol requires cleavage in the transmembrane region by a protease activity that is specifically blocked by a novel cysteine protease inhibitor. (2000) J. Biol. Chem. 275(40):30951-6. Abstract pdf

Wang EW, Kessler BM, Borodovsky A, Cravatt BF, Bogyo M, Ploegh HL, Glas R Integration of the ubiquitin-proteasome pathway with a cytosolic oligopeptidase activity. (2000) Proc Natl Acad Sci 97(18):9990-5. Abstract pdf

Greenbaum D, Medzihradszky KF, Burlingame A, Bogyo M Epoxide electrophiles as activity-dependent cysteine protease profiling and discovery tools. (2000) Chem Biol 7(8):569-81. Abstract pdf

Caffrey CR, Mathieu MA, Gaffney AM, Salter JP, Sajid M, Lucas KD, Franklin C, Bogyo M, McKerrow JH Identification of a cDNA encoding an active asparaginyl endopeptidase of Schistosoma mansoni and its expression in Pichia pastoris. (2000) FEBS Lett 466(2-3):244-8. Abstract pdf

Bogyo M, Verhelst S, Bellingard-Dubouchaud V, Toba S, Greenbaum D Selective targeting of lysosomal cysteine proteases with radiolabeled electrophilic substrate analogs. (2000) Chem Biol 7(1):27-38. Abstract pdf supplemental materials


1999
Schmidtke G, Holzhutter HG, Bogyo M, Kairies N, Groll M, de Giuli R, Emch S, Groettrup M How an inhibitor of the HIV-I protease modulates proteasome activity. (1999) J Biol Chem 274(50):35734-40. Abstract pdf

Selzer PM, Pingel S, Hsieh I, Ugele B, Chan VJ, Engel JC, Bogyo M, Russell DG, Sakanari JA, McKerrow JH Cysteine protease inhibitors as chemotherapy: lessons from a parasite target. (1999) Proc Natl Acad Sci 96(20):11015-22. Abstract pdf


1998
Bogyo M, Ploegh HL Antigen presentation. A protease draws first blood. (1998) Nature 396(6712):625-627. Abstract pdf

Bogyo M, Shin S, McMaster JS, Ploegh HL Substrate binding and sequence preference of the proteasome revealed by active-site-directed affinity probes. (1998) Chem Biol 5(6):307-20. Abstract pdf

Glas R, Bogyo M, McMaster JS, Gaczynska M, Ploegh HL A proteolytic system that compensates for loss of proteasome function. (1998) Nature 392(6676):618-22. Abstract pdf

Ruepp A, Eckerskorn C, Bogyo M, Baumeister W Proteasome function is dispensable under normal but not under heat shock conditions in Thermoplasma acidophilum. (1998) FEBS Lett 425(1):87-90. Abstract pdf


1997
Bogyo M, McMaster JS, Gaczynska M, Tortorella D, Goldberg AL, Ploegh H Covalent modification of the active site threonine of proteasomal beta subunits and the Escherichia coli homolog HslV by a new class of inhibitors. (1997) Proc Natl Acad Sci 94(13):6629-34. 9316392 Abstract pdf

Bogyo M, Gaczynska M, Ploegh HL Proteasome inhibitors and antigen presentation. (1997) Biopolymers 94(13):6629-34. Abstract pdf


1996
Wiertz EJ, Tortorella D, Bogyo M, Yu J, Mothes W, Jones TR, Rapoport TA, Ploegh HL Sec61-mediated transfer of a membrane protein from the endoplasmic reticulum to the proteasome for destruction. (1996) Nature 384(6608):432-8. Abstract pdf

Wiertz EJ, Jones TR, Sun L, Bogyo M, Geuze HJ, Ploegh HL The human cytomegalovirus US11 gene product dislocates MHC class I heavy chains from the endoplasmic reticulum to the cytosol. (1996) Cell 84(5):769-79. Abstract pdf


1994
Wenzel T, Bogyo M, Lebeau E Lanthanide-cyclodextrin complexes as probes for elucidating optical purity by NMR spectroscopy. (1994) J. Am. Chem. Soc. 116:4858-65.pdf